RESUMO
This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 ± 7.73% fibrin only and 21.11 ± 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 ± 8.89%, p < 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group (p Ë 0.05). The areas of myofiber formation in the fibrin only group (31.77 ± 10.81%) and fibrin with laminin-nidogen group (43.13 ± 10.39%) were larger than in the empty wounds (28.10 ± 11.68%, p Ë 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.
Assuntos
Fibrina/genética , Fibrose/genética , Palato Mole/lesões , Cicatrização/genética , Actinas/genética , Animais , Colágeno/genética , Fibrina/farmacologia , Fibrose/patologia , Fibrose/terapia , Humanos , Laminina/genética , Laminina/farmacologia , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/farmacologia , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/crescimento & desenvolvimento , Miofibrilas/genética , Cadeias Pesadas de Miosina/genética , Fatores de Transcrição Box Pareados/genética , Palato Mole/efeitos dos fármacos , Palato Mole/patologia , Ratos , Regeneração/genéticaRESUMO
OBJECTIVE: The aim of this study was to analyze the function of the palatal muscles in vivo by real-time wireless electromyography in rats. The effects of palatal wounding were also analyzed. METHODS: Microelectrodes were implanted six rats; in the masseter muscle (two-rats) for comparison, in the unwounded soft palate (two-rats) and the soft palate that received a surgical wound (two-rats). Two weeks after implantation, a wound was made in the soft palate using a 1â¯mm biopsy-punch. Electromyographic measurements and video-recordings were taken weekly to monitor train-duration and peak-amplitude during eating, grooming and drinking. RESULTS: The train-duration of the masseter muscle during eating was 0.49⯱â¯0.11â¯s (rat-1) and 0.56⯱â¯0.09â¯s (rat-2), which was higher than during grooming. In the unwounded soft palate the train-duration during eating was 0.63⯱â¯0.12â¯s (rat-1) and 0.69⯱â¯0.069â¯s (rat-2), which was higher than during grooming and drinking. The peak-amplitude for eating in the normal soft palate before surgery was 0.31⯱â¯0.001â¯mV (rat-1) and 0.33⯱â¯0.02â¯mV (rat-2). This decreased to 0.23⯱â¯0.03â¯mV and 0.25⯱â¯0.11â¯mV respectively, after surgery. For drinking the peak-amplitude was 0.30⯱â¯0.01â¯mV (rat-1) and 0.39⯱â¯0.01â¯mV (rat-2) before surgery, which decreased to 0.23⯱â¯0.09â¯mV and 0.20⯱â¯0.14â¯mV respectively, after surgery. CONCLUSION: The reduced peak-amplitude suggests impaired soft palate function after wounding. This is the first study into the in vivo function of the soft palate after surgical wounding. This model will contribute to develop strategies to improve soft palate function in patients.
Assuntos
Músculos Palatinos/fisiologia , Palato Mole/fisiologia , Ferimentos e Lesões/fisiopatologia , Animais , Eletromiografia , Músculo Masseter/fisiologia , Palato Mole/lesões , RatosRESUMO
Introducción: Las enfermedades del músculo esquelético en las que la lesión supera la capacidad deadaptación conducen a cambios patológicos propios de las miopatías. En Colombia el diagnóstico serealiza mediante tinción con hematoxilina-eosina; sin embargo, la información brindada sobre algunosde los cambios del tejido muscular es limitada, por lo que es necesario realizar algunas técnicas de histoquímicaenzimática según la alteración. Objetivo: Describir los criterios de tinción de diferentes técnicashistoquímicas en el diagnóstico de las miopatías. Materiales y métodos: Se realizó una revisiónsistemática mediante la búsqueda de artículos en inglés y español indexados en las bases de ISI Web ofScience, Ovid Medline, PubMed, ScienceDirect y EBSCO. Los descriptores MeSH utilizados fueron:«histochemistry¼, «skeletal muscle¼, «structural and congenital myopathies¼, «fiber type fast twitch¼,«fiber type slow twitch¼ y «NADH tetrazolium reductase¼. Se asociaron a la búsqueda los términos«clinical application¼ y «diagnosis¼. Se seleccionaron los artículos originales, reportes de casos y revisionespublicadas entre 1982 y 2014 que aplicaran las técnicas de histoquímica en el diagnóstico de unamiopatía o en la investigación biomédica. Resultados: Se seleccionaron 64 publicaciones (incluidosdos libros) que fueron representativos para la redacción de este manuscrito, el cual fue complementadocon imágenes de biopsias musculares procesadas en el Laboratorio de Histología de la Universidad delValle, Colombia...
Introduction: The injuries on skeletal muscle that go beyond their capacity to adaptation result incharacteristic pathological changes of the myopathies. In Colombia for their diagnosis is usually usedthe hematoxylin-eosin stain, however, the information about many changes in muscle tissue is limited.That is why it is necessary to carry out certain enzymatic histochemical staining according to eachmyopathy. Objective: To describe the different criteria to diagnose myopathies by histochemical techniques.Material and methods: A systematic review was conducted which focused on search of all articleswritten in English and Spanish indexed in ISI Web of Science database, Ovid Medline, PubMed,ScienceDirect, and EBSCO. The MeSH descriptors used for the search were «histochemistry¼, «skeletalmuscle¼, «structural and congenital myopathies¼, «fast twitch fiber type¼, «slow twitch fiber type¼,and «NADH tetrazolium reductase¼. The terms «clinical application¼ and «diagnosis¼ were associatedto the search. All original articles, case reports, and reviews published during 1982-2014, which imple-mented histochemical techniques to the diagnosis of myopathy or to biomedical research were included.Results: From systematic search, 64 publications (including two books) were representative for thedrafting of this manuscript. The article was supplemented with images from muscle biopsies processedby the Laboratory of Histology, Universidad del Valle, Colombia...
